Arsenal, who secured the title last weekend, ended their campaign with a 4-1 win against Birmingham City.
Little's tremendous goal-filled season ended with her 22nd in the league and her 47th in all competitions.
First-half strikes by Lauren Bruton and Jennifer Beattie set the Gunners up for a comfortable victory over the third-bottom team.
Little added the third goal seven minutes after the interval and captain Jayne Ludlow bagged the fourth four minutes from time.
Substitute Gina Goulding hit a last-minute consolation effort, but Birmingham's season limped to a sad end with their sixth defeat in seven outings.
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