Arsenal Ladies have been drawn in the same group as Liverpool, Lincoln and Chelsea in the FA WSL Continental Cup.
Laura Harvey's team won the inaugural competition last year but will need to master a new format to successfully defend their trophy.
The Continental Cup now features two groups of four teams with the top two advancing to the semi-finals. The final will take place at the end of the 2012 season in October.
Arsenal kick off their Continental Cup quest at Liverpool over the weekend of March 17 and 18 before travelling to Lincoln on May 12 or 13.
Their final group game is at home to Chelsea on June 9 or 10.